pkh67 green fluorescent cell linker mini kit for general cell membrane labeling  (Merck & Co)

Journal: Cell Reports Medicine

Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy

doi: 10.1016/j.xcrm.2026.102632

Figure Lengend Snippet: Identification of Val-ILs affecting T lymphoma growth (A) Transmission electron microscopy imaging showing a representative image of Val-ILs. Magnifications, ×94K (left) and ×310K (right); scale bars: 100 nm (left) and 50 nm (right). (B) Representative quantification and size of Val-ILs measured by NTA. (C) C57BL/6 mice were s.c. injected with 10 6 EL4 cells, into the side of the body. When tumors reached 50 mm 3 , mice were treated with Val-ILs i.v. injected on days 6 and 9 (10 12 NPs). A screening of 31 NPs loaded with different antibodies identified nine NPs that exhibited a strong tumor volume reduction by day 12 (pink bars). (D) Mice were treated with Val-ILs (10 12 NPs) on days 6, 9, and 12. The efficacy of the nine individual Val-ILs, as well as the combined Val-ILs targeting the nine identified antigens (Val-ILs-Combo), was evaluated on day 12 (left panel) and over time (right panel), n = 4 mice per group, two independent experiments performed. (E) Mice were i.v. injected with trackable NPs labeled with the PKH67 green fluorescent dye, n = 4 mice. The proportion and the number of PKH67 + NPs detected by FC in different tissues, after 18 h. PKH67 + NPs were detected in the spleen, the lymph nodes, and the bone marrow, whereas undetected (ud) in the blood, the thymus, the kidney, and the tumor. UV, unilamellar vesicle. (F) Mice were i.v. injected with Val-ILs labeled with PKH67. Cells were extracted 18 h later from various tissues and analyzed by FC, n = 5 mice per group. Data showing that trackable NPs bound to immunosuppressive cells in the spleen and the TDLNs. MFI, mean fluorescence intensity. Data are shown as means ± SD, p values are compared to Val-ILs-IgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .

Article Snippet: PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling , Merck , Cat# MINI67.

Techniques: Transmission Assay, Electron Microscopy, Imaging, Injection, Labeling, Fluorescence

Journal: ADMET & DMPK

Article Title: Anti-inflammatory potential of plant-derived extracellular vesicles from Solanum nigrum L. integrated in gelatine-dopamine hydrogel on RAW 264.7 and MC3T3 cells

doi: 10.5599/admet.3149

Figure Lengend Snippet: Uptake of plant-derived extracellular vesicles from Solanum nigrum L. berries by (A) RAW 264.7 and (B) MC3T3 cells after 12 hours of incubation using a confocal microscope. Uptake of PKH67-labeled PDEV is shown by green colour and DAPI-stained cell nucleus is shown by blue colour

Article Snippet: To evaluate the uptake of PDEV into MC3T3 and RAW 264.7 cells, PDEV released from the hydrogel after 24 hours was first fluorescently labelled using the PKH67 Green Fluorescent Cell Linker kit (Merck) according to the manufacturer's protocol.

Techniques: Derivative Assay, Incubation, Microscopy, Labeling, Staining